Profil Aktivitas Antioksidan Ekstrak Polar dan Non-Polar Umbi Porang (Amorphophallus muelleri Blume) Menggunakan Uji DPPH

Abstract

Porang tuber (Amorphophallus muelleri Blume) is an Indonesian native commodity rich in glucomannan polysaccharides and potentially serves as a natural antioxidant source to counteract free radicals that trigger degenerative diseases. Objective of the researchv to map the antioxidant activity profile of non-polar, semi-polar, and polar extract s of porang tuber and determine the IC50 value using the DPPH method. Porang tuber powder was extracted through multilevel maceration using n-hexane, chloroform, ethyl acetate, and ethanol solvents. Antioxidant activity was tested using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method with UV-Vis Spectrophotometry at 517 nm, utilizing Vitamin C and Quercetin as positive controls. Result: The results showed that all extract possessed extremely strong antioxidant activity. The lowest IC₅₀ value was obtained from the ethyl acetate extract at 3.44 ppm, followed by the ethanol extract at 3.61 ppm and the n-hexane extract at 3.81 ppm. The activity of the ethyl acetate extract was stronger than the positive controls, Vitamin C (3.75 ppm) and Quercetin (4.52 ppm). Multilevel extraction is effective in separating the active compounds of porang tuber, with the semi-polar extract (ethyl acetate) exhibiting the most optimal and extremely strong antioxidant potential.